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Results: 164 out of 400 patients completed the survey (41%) with a mean age of 64 10 years, 54% male and 75% non-Hispanic white

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After release of the protoplasts with a pipette, the suspension was transferred to a filter-sterilized MSC solution (10 mM MES pH 5.8, 0.4 M sucrose, 20 mM MgCl 2 .6H 2 O, 467 mM mannitol) and overlaid with MMM solution

Balancing sedimentation-induced gravity by centrifugation (horizontally at 30 rpm for 24 h) was carried out to generate the simulated microgravity 42