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Pala, O., Diaz, A., Blomberg, B

26 , 672685.e674 (2017)

In a study on aging-related diseases, an increase in the abundance of gut microbiome genes involved in the CoQH 2 synthesis pathway was observed with age [124]

Conversely, miR-23a-3p targets cul3, a principal regulator of Nrf2 ubiquitination and degradation, thereby further increasing the activity of Nrf2

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]