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This process can be initiated by iron-catalyzed Fenton chemistry, in which hydroxyl radicals (OH) generated from H 2 O 2 trigger a self-propagating lipid peroxidation chain reaction

This elution step works because the free glutathione competes with the immobilized glutathione for binding to GST, thereby releasing the fusion protein from the column

Crit Rev Oncol Hematol (2021) 160:103281