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Molecules 27, 901

doi: 10.1128/JVI.65.11.5757-5764.1991 101 KanaiRWakimotoHMartuzaRLRabkinSD

In general, single-end sequencing is sufficient in case of well-annotated organisms and frequently focuses only on mRNAs, while pair-end sequencing is recommended for de novo transcript discovery or isoform analysis