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CTAs use cultured mammalian cells to measure their ability to undergo malignant transformation in response to a test substance ( Although CTA is considered insufficient for classifying chemicals as carcinogens on its own, it is a crucial component integrated approach to testing and assessment (IATA) for non-genotoxic carcinogens (NGTxC) based on leveraging omics technology, particularly transcriptomics, to gain a more nuanced mechanistic understanding of the behavior exhibited by the tested chemical ( in vitro models for testing chemically induced transformations, which have been considered for inclusion in the IATA for NGTxC: the SHE model, BALB/c 3T3 model, and Bhas42 CTA, differing in the degree of cell progression towards transformation

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