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Viele Produktionsmethoden basieren auf Chemikalien, Hitze und/oder hohem Druck

Total ROS and mtROS measurements Total ROS and mtROS were measured using the fluorescent probes H2DCFDA (Thermo Fisher Scientific, Waltham, MA, USA) and MitoSOX (Thermo Fisher Scientific, Waltham, MA, USA), respectively

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Subsequently, they were stained with Annexin-V conjugated with FITC and Propidium iodide (PI) (Vazyme, A211-0) for 10 min at room temperature to detect apoptosis following the products instructions

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