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The actual cell lysis was then carried out using a PBS wash step before the addition of 0.5 ml of 1x RIPA lysis buffer (50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% DOC, and 0.1% SDS) supplemented with 1x protease inhibitor, to the cells

Zhu Y et al (2024) Carrier-free self-assembly nano-sonosensitizers for sonodynamic-amplified cuproptosis-ferroptosis in glioblastoma therapy

Thiols such as glutathione, alpha lipoic acid and NAC are powerful sulfur bearing antioxidants

In addition, activated Akt can relinquish the inhibition of mammalian target of rapamycin (mTOR) signaling pathways and promote proteins synthesis (Fig

(2010) RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila