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All lenses were incubated for 3 to 4 days in 2 mL of M199 medium (Sigma-Aldrich) containing 0.1% BSA and 30 mM Galactose using an incubator set at 5% CO 2 and 37 C to induce opacity, as previously reported 30

To reduce BAC levels responsibly, stop drinking well before driving or operating machinery, stay hydrated with non-alcoholic beverages, engage in physical activity to boost metabolism, and avoid mixing alcohol with medications or other substances that can enhance its effects

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Subcellular distribution of the enzymes of the glutamic acid, glutamine and -amino-butyric acid cycles in rat brain