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doi: 10.1007/s00401-015-1440-1 104

PLoS One 2012

Primary antibodies used were rabbit anti-HO-1 (1:1000), mouse anti-NQO-1 (1:1000), mouse anti-p62/SQSTM1 (1:1000) and mouse anti-actin (1:5000) (see antibody characterization below and in Supplementary Table S1)

The hydrolysis of peptide bonds is essential for analyzing amino acids in peptides and proteins, as it forms free amino acids, which can then be analyzed