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Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation

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Materials and methods Materials Nunc EasYFlask 225 cm 2 (T225) cell culture flasks, Corning Costar six-well clear tissue culture-treated flat bottom plates, Falcon 50 mL conical centrifuge tubes, Basix microcentrifuge tubes, Invitrogen Countess cell counting chamber slides, Nunc 25 mL serological pipettes, Greiner Bio-One CELLSTAR 24-well cell culture plates, Corning 96-well solid white non-sterile microplates, 9 and 5 glass Pasteur pipets, Gibco trypsinEDTA (0.25%) phenol red, Gibco DMEM/F-12 with l-glutamine and phenol red, Corning penicillinstreptomycin (pen-strep) solution (10,000 IU penicillin, 10,000 g/mL streptomycin), and Corning regular fetal bovine serum (FBS) were purchased from Fisher Scientific (Pittsburgh, PA, USA)

B.J.C.W was supported by the Training Program in Quantitative and Chemical Biology (QCB) at Indiana University (T32 GM131994).

The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products