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1 Trial design for STEP 5 clinical study

The full blood count test will also include a level called the mean cell volume (MCV), which is raised with vitamin B12 deficiency but also in some other conditions

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Orthogonal validation of NMR fragment hit 27 Encouraged by the relatively high binding affinity of compound 27 compared to expectations for fragments, we synthesised a series of analogues to explore the effect of modifying both the sulfonic acid and the ethoxy substituents on binding affinity (Table 1)