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More recently, dual AAV-LNP approaches for prime editing have shown promise: scAAV-delivered pegRNAs combined with LNP-encapsulated PE7 mRNA achieved 20.7% correction of the Pah enu2 mutation in phenylketonuria mice, reducing blood phenylalanine below therapeutic thresholds [315]

The HBEC cells were cultured in K-SFM, and the other cells were cultured in DMEM essential medium with 10% fetal calf serum at 37 C in a humidified incubator in an atmosphere of 95% air and 5% CO 2

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We used thresholding for the quantification of TfR1 downregulation (Figure 2)

The elution buffer from your affinity step is rarely optimal for proteolysis