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Briefly, total RNA from cells was isolated using a Pure link RNA Mini kit (Life Technologies, USA), according to the manufacturers protocols

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Additionally, HD has been shown to protect against Valproate-induced hepatotoxicity by its scavenging of free radicals, antioxidant property and prevent oxidative stress mediated apoptosis, as assessed by liver marker enzymes, oxidative stressors, antioxidant enzymes, mRNA and protein expression of apoptotic proteins (Nathiya et al., 2016)