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The mTeSR medium was supplemented with 10 M of p160ROCK inhibitor Y-27632 (Tocris, Minneapolis, MN) during the first 24 h after thawing

The anorectic effects of GLP-1 agonism are reported to result from the suppression of AgRP/NPY neurons and activation of POMC/CART neurons, and these responses occur upstream of MC4R [28, 29]