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The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes

In further support of this hypothesis, it is worth noting that prior research has demonstrated that proteasomal degradation decreases at higher oxidant levels (Breusing and Grune 2008), and that the down-regulation of those proteins involved in protein degradation that were included within the top ten list became more pronounced from day 6 to day 8

Authors:Sullivan DM, Levine RL, Finkel T, Journal:Methods Enzymol PubMed ID:12078486 Detecting oxidative post-translational modifications in proteins

Hanaoka, K., Sasakura, K., Suwanai, Y., Toma-Fukai, S., Shimamoto, K., Takano, Y

[DOI] [PubMed] [Google Scholar] 19.Richie JP, Jr, Abraham P, Leutzinger Y