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Meanwhile, plasmid pYES2/CT (Invitrogen, Carlsbad, CA, United States) was first digested with Eco RI and Xba I, gel-purified, and ligated with the above two products using ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China) and then transformed into Escherichia coli DH5a cells following the manufacturers instructions

Exploring these applications could broaden the therapeutic scope of rhamnetin

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