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One-step reverse transcription-qPCR (real-time PCR) was performed in accordance with methods described by the manufacturer and previous publications [46,47]

47 Taken together, these data suggest the reduced GSH/GSSG antioxidant capacity is insufficient to counter excessive OH production, and that unopposed OH can reach the nucleus to create the oxidative DNA adduct, 8-oxo-dG

There is a consensus that GPX and GCL are the two enzymes in the glutathione pathway that are upregulated by estradiol

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Our work demonstrates that the response to exogenous glucose in the brain is also impaired in GLP-1R KO mice