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Slides were blocked in blocking solution [2% (w/v) BSA, 0.1% (v/v) Tween 20 in PBS] for 20 min and incubated with primary antibodies: rat monoclonal anti-BrdU (Abcam, ab6326

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We acknowledge the utilization of this flexibility and recognize that many practitioners have stressed the importance of maintaining it, however we seek additional information regarding potential patient safety and quality of care concerns

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