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Then, 96-well plates were read in a fluorescence plate reader (Molecular Devices, SpectraMax Gemini XPS) in an excitation wavelength of 544 nm and an emission wavelength of 590 nm

lysis buffer 5 mM imodazole in lysis buffer imidazole 5 mM and 20 mM, in lysis buffer Bound mt-hNNMT was eluted from the column with lysis buffer and 150 mM imidazole, 200 mM salt, and 5% glycerol in 1 ml aliquots

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