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In contrast, the gdh3 / gdh3 mutant showed overall higher concentrations of intracellular metabolites than the wild-type strain when grown in MM medium, except for 2-aminobutyrate and norvaline.When grown in a proline medium, the gdh3 / gdh3 mutant revealed a significantly higher concentration of glutamate family metabolites (e.g., glutamate, glutamine, and proline) than the wild-type, whilst asparagine, histidine, phenylalanine, valine, leucine, tyrosine, and aspartate family metabolites (e.g., aspartate, isoleucine, and threonine), as well as all saturated fatty acids detected, were at lower concentrations in the gdh3 / gdh3 mutant

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According to a previous analysis of tear fluid from 18 normal controls and 36 PD patients using mass spectrometry, PD patients had considerably greater amounts of 21 proteins and lower levels of 19 proteins than those of the normal subjects