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This protocol is an extension of the widely accepted PEN-FAST (penicillin allergy reported by patient, five years or less since reaction, anaphylaxis or angioedema, severe cutaneous adverse reaction, and treatment required for reaction) clinical decision tool that has been used to identify patients with reported penicillin allergy who are appropriate for DOC rather than immediate hypersensitivity skin testing, which recent data suggest has poor positive predictive value in low-risk patients

Sulfa drugs (also called sulfur medications or sulfonamide-containing drugs) are drugs that contain a sulfonamide functional group in their compound structure

Immunocytochemistry Prior to the start of experiments, glass cover slips (Bellco Glass Inc., Vineland, NJ, USA) were coated in 24-well plates (Thermo Fisher Scientific) with PLO and FN before seeding the cells

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doi: 10.1021/acssuschemeng.4c08602 78 NaczkMShahidiF