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Cells were plated in 6-well plates at 7 10 5 cells per well and transfected with 2.5 g of the appropriate TCR or CAR construct in the retroviral vector pMP71 using Lipofectamine 3000 (Life Technologies, Invitrogen)

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These processes include cancer initiation, promotion, and progression, and are regulated through mechanisms such as cell proliferation, differentiation, apoptosis, angiogenesis, and metastasis