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Listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells

If youre working with an uncharacterized protein, or a protein for which a good antibody has not been developed (and just because your protein has a commercially available antibody, that doesnt mean its a good one), then your first step towards detecting, immunoprecipitating, or purifying that protein may be to fuse an affinity tag to it

Gutman made another career shift, committed to advancing glutathione research, eventually becoming both the personal physician of and trusted collaborator with Dr

A novel oxidoreductase family sharing a conserved FAD-binding domain

Cell viability Cell viability was assessed by a colorimetric assay where MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 0.5 mg/ml