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Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C

It is important to remember that lipoproteins and other lipid material can rapidly clog chromatography columns, so it may be necessary to remove them before purification using precipitation agents like dextran sulfate or polyvinylpyrrolidone