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In these individuals, early development is often marked by hypotonia, feeding difficulties, limited babbling, delayed onset of first words, and marked difficulty in acquiring speech which is unintelligible in the preschool years, when a diagnosis is usually made [4]

S21374, Invitrogen) in 0.4% Triton-100 at RT

Statistical Analysis All experiments were repeated at least three times and the data were presented as mean SEM

While this approach offers important mechanistic insights, it does not fully reproduce the complexity of the in vivo metabolic environment, where interactions with glial and other cell types are critical

Jansson, A., and Dahlborn, K