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doi: 10.1038/npp.2013.274 92 Kasteleijn-Nolst TreniteD.ParainD.GentonP.MasnouP.SchwartzJ

Treatment comprised leukapheresis, cell isolation and maturation with reinfusion of macrophages to a maximal concentration of 1 x 10 9 cells

This is done while the patient is awake in the office, under a local anaesthetic

After 48 h, cells were pelleted at 2000 g and resuspended in breaking buffer (50 mM sodium phosphate, 5% glycerol, 1 mM EDTA, pH 7.4) and frozen prior to processing