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20 Detection Methods There are numerous methods for detecting glutathione, with commonly used ones including the iodometric method, the DTNB (Ellman's reagent) method, the sodium nitroprusside method, the fluorometric method, the alloxan method, and high-performance liquid chromatography (HPLC)

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This process results in increased contents of the antioxidant enzymes SOD, catalase (CAT), and glutathione (GSH), as well as a decreased MDA content, thereby inhibiting oxidative stress

These findings, together with the widespread expression of UBE3A in mammalian tissues including the SCN, suggest a conserved role for UBE3A in circadian timekeeping across species (Tian et al., 2011