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As demonstrated recently by Liu et al ., an increase in labile iron was required for ferroptosis triggered by cysteine deficiency but not direct inhibition of GPX4 [51]

The author claimed that the position of the last double bond is more critical for induction of ferroptosis than the degree of unsaturation

S11223), and streptavidin Alexa Fluor 555 (Thermo Fisher Scientific

Next, the membrane was incubated with electrochemiluminescence substrate (#1705060, Bio-Rad, Hercules, CA, USA) for 5 min and analyzed using FluorChem M (ProteinSimple, Santa Clara, CA, USA)

The cell suspension was subsequently transferred to a flat-bottomed 96-well plate, with 200 l per well