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Multi-photon and second harmonic generation microscopy The collagen gels were fixed with 4% paraformaldehyde for 20 minutes and washed in PBS with 0.1% saponin and 0.1% Tween 20 before being incubated with phalloidin conjugated with Alexa Fluor 594 for 1 h at room temp

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Liraglutide (Saxenda), administered as a daily 3.0 mg subcutaneous injection, was the first GLP-1 agonist approved specifically for weight management and produces average weight loss of 5-10% in clinical trials