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Upon activation, NLRP3 engages the scaffold protein NIMA related kinase 7 (NEK7) and recruits the adaptor apoptosis-associated speck-like protein containing a CARD (PYD and CARD domain containing (PYCARD, also known as ASC)) to assemble the inflammasome complex

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If results are weak into the remodelling phase and starting dose was sub-3.2 mg, a dose increase and protocol modification may be warranted