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Rettenmeier AW, Prickett KS, Gordon WP, Bjorge SM, Chang SL, Levy RH, et al

This mixture was incubated for 30 minutes, then acetic acid was added, and the mixture was further incubated in a hot water bath for 3 minutes

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Briefly, the 4-NP dissolved in water was mixed with NaBH 4 , and then yellow color nitrophenolate anion solution was formed with a characteristic absorbance peak at 400 nm