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Determination of MMP To determine MMP, cells were incubated with TMRM (50 nM

Medical Conditions: Inform your healthcare provider about any preexisting medical conditions, such as kidney or heart disease, as these may affect the suitability of HCG treatment

It also increased GSH and decreased MDA and NO after seven days of intermittent fasting, which may be due to a decrease in oxidative damage

While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain