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Borsini, F

We show that the addition of CellTox Green Dye to the cells does not have a significant effect on the subsequent luminescent signal generated from the GSH/GSSG-Glo Assay

About this article Cite this article Zhang, Q., Burrell, J.C., Zeng, J

Sanso, M

Affinity purification handles can be divided into two groups: (1) peptides or proteins that bind a small ligand immobilized on a solid support, as for instance, the His 6 tag and nickel affinity resins, and (2) tags that bind to an immobilized molecule such as antibodies ( The purification of a target protein using an affinity handle offers several advantages over the conventional chromatographic methodologies, namely: (i) The target protein never interacts directly with the chromatographic resin (Waugh, 2005)