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4.5 Immune system Typically, during the last months of gestation and the first month of lactation, there is an increase in fat mobilization from maternal depots and an accumulation of hepatic non-esterified fatty acid (NEFA) (Pullen et al., 1990)

Although efforts have been made to develop glycolysis-targeting agents, inhibitors of HIFs, and immune checkpoint inhibitors for treating GBM, all therapeutic approaches present elevated toxicity without improvement in the outcomes of patients with GBM [13,14,15,16,17]

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A key objective for Lefkovits with the experiment was to get non-responding cultures as this was key to being able to calculate the number of precursor cells involved in antibody formation using the Poisson distribution equation (Lefkovits 2025)

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