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10.1074/mcp.O116.065219 39 UzawaS.YamanoY.TakiguchiY.TanzawaH.TatsumiK.UzawaK

9 December 2021

The associations of IGF2, IGF2R and IGF2BP2 gene polymorphisms with gestational diabetes mellitus: A case-control study

Constructs were cloned into a pET21a vector (MilliporeSigma, Burlington, MA) using Gibson Assembly cloning 66 , and plasmids transformed into Rosetta2 cells (MilliporeSigma, Burlington, MA) or BL21 cells (Invitrogen, Carlsbad, CA)