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For PCR analysis, 2 L of the lysate was used as a template with the following primers: F1: 5-GCCAGCTACTGCATATATTGAGACC-3 R1: 5-GGATATAGAGAAGCCCTAGAACAGGG-3 R2: 5-CATGTCGTGTTGGTCAAATAAGCAC-3 PCR amplification using primers F1/R1 generated a 278 bp product for wild-type (WT) alleles and a 382 bp product for floxed alleles

Biochim Biophys Acta 1275 , 161203 (1996)

doi: 10.1016/j.gtc.2007.12.006

Electrical stimulation accelerates motor functional recovery in the rat model of 15-mm sciatic nerve gap bridged by scaffolds with longitudinally oriented microchannels

( 5 ) Normal Testosterone Levels by Age Testosterone peaks in early adulthood and gradually declines as men age