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The column was held at 40 C

This refers to the specific modifications made to the standard IGF-1 moleculean Arginine replacing a Glutamic Acid at the 3rd position and a 13 amino acid extensionto extend its half-life

However, studies have shown that it acts directly on the pituitary gland and the hypothalamus, as well as GHRH and somatostatin

Garzn, Revisiting the scavenging activity of glutathione: Free radicals diversity and reaction mechanisms, Computational and Theoretical Chemistry, Volume 1227, 2023, 114227, ISSN 2210-271X