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Essentially the tissue is damaged and sensation in the area may be lost

First separation was carried out with 15 mM ammonium bicarbonate in 90% water and 10% acetonitrile as mobile phase A, and mobile phase B was 15mM ammonium bicarbonate in 95% acetonitrile and 5% water

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This is why we identify the result as chromatographic purity by HPLC rather than simply listing purity. We believe researchers should understand exactly what is being measured and how that result should be interpreted