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Step 3 Reconstitution Insert the needle into the BPC-157 + TB-500 vial Inject the water slowly along the vial wall (avoid spraying directly on the powder) Remove the syringe and swirl gently until dissolved ( never shake) Let the vial rest for 35 minutes until the solution clears

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From day 7 to day 10 the differences between control and AITC-treated seedlings were smaller than those observed during the second period, indicating that the effect of AITC on root growth persisted until day 10 but decreased over time (Supplementary Figure S2)

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