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This pathogenic process is further influenced by oxidative and endoplasmic reticulum stress, GM dysbiosis, disruptions in genetic and epigenetic regulation, mitochondrial dysfunction, as well as various environmental and dietary determinants [8]

Since the cellular synthesis of glutathione is ATP-dependent, decreased ETC activity also undermines glutathione synthesis, further exacerbating oxidative stress in a vicious cycle [8]

Varying amounts of monobromobimane stock solution (0-300 l) were added to respective cuvettes already containing 5 l of the enzyme solution (protein concentration of 810 g ml -1 ), glutathione (100 l of stock solution) and phosphate buffer (3.0 ml)

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Theor Appl Genet 91:802808 Traka M, Gasper AV, Smith JA et al (2005) Transcriptome analysis of human colon Caco-2 cells exposed to sulforaphane