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For glutathionylation experiments, 30 10 6 cells were washed with RPMI supplemented with 0.5% FBS and 100 U/mL penicillin-streptomycin and resuspended in a total volume of 5 mL of the same medium

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In addition, the large surface of proteins involved in the interaction among proteins is not covered by small molecules because their sizes are too small 124

Cellular GSH is found mostly in the cytosol, with the remainder spread across organelles ( The thioredoxin system is composed of Trx proteins and Trx reductases (TrxR), in which the reduced form of Trx proteins (Trx(SH) 2 ) are disulfide reductases as they contain dithiol groups in their highly conserved active site (-Cys-Gly-Pro-Cys-) ( 2 can directly reduce oxidized proteins or provide electrons to thiol-dependent peroxidases (Prx) to convert hydrogen peroxide to water