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Pharmacologic ascorbate (P-AscH(-)) suppresses hypoxia-inducible Factor-1alpha (HIF-1alpha) in pancreatic adenocarcinoma

C.ZhangJ.JuddR

About 2.5 kg of the powdered plant material was extracted successively by cold maceration method with different solvents (10 L each for 2436 h) of increasing polarity (petroleum ether, chloroform, ethyl acetate and ethanol)

A large panel of cytokines, chemokines and growth factors was measured using a pre-defined, commercially available, Luminex kit from Millipore (Burlington, MA) which included the cytokines interleukin (IL)-1, IL-1, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-15, IL-17A, interferon (IFN)-2, IFN-, tumor necrosis factor (TNF)-, TNF-, granulocyte colony-stimulating factor (GCSF), granulocyte-macrophage colony-stimulating factor (GMCSF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP1/CCL2), IFN-induced protein/chemokine (C-X-C motif) ligand 10 (IP10/CXCL-10), macrophage inflammatory protein-1 alpha (MIP1/CCL3), MIP-1 beta (MIP1/CCL4), and eotaxin (CCL11)

28 DelareP.DuyckaertsC.MastersC.BeyreutherK.PietteF.HauwJ