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G., Roila, F., de Wit, R., & Carides, A

Determination of choline, betaine, and dimethylglycine in plasma by a high-throughput method based on normal-phase chromatography-tandem mass spectrometry

Wherever possible, calibrate with defined oxidant fluxes (enzymatic generators), internal standards, or ratiometric reporters and fully report matrix and environmental conditions (buffer composition, protein content, temperature, pH, oxygen tension, light exposure) plus acquisition timing/kinetics to separate bursts from steady states (Kalyanaraman et al