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Cells were incubated for 10 min at room temperature and then 20 L of 10% CHAPS detergent (Sigma-Aldrich, C3023) was added, and cells incubated at room temperature for an additional 10 min to lyse cells

These integrative approaches combine nutritional optimization, exercise and movement strategies, sleep and circadian rhythm optimization, stress management and psychological wellbeing, targeted supplementation including glutathione, hormone optimization where appropriate, and regular monitoring of health biomarkers and age-related changes

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In the clinical context, it is frequently observed that infertile women undergoing ART treatment often express concerns about potential side effects, including the development of breast cancer