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Description

Ammonium sulfate treatment Crude extract was gradually saturated with 40% (NH 4 ) 2 SO 4 with continuous stirring (30 min, 4 C) followed by centrifugation (8000 g , 20 min)

10.15252/embr.202052280 57 YangC.WangT.ZhaoY.MengX.DingW.WangQ.et al (2022)

doi: 10.1074/jbc.M110.158055 104 YerkovichSTRoponenMSmithMEMcKennaKBoscoASubrataLSet al

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In order to test this hypothesis, we pre-treated serum samples with a protein G/L bead or a sepharose control bead and tested the samples again in the TNF immunoassay