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Cell supernatants (up to 200 g of glycoprotein in a volume of 35 l) were denatured with 10 l of 250 mM phosphate buffer and 2.5 l of 2% SDS with 1 M 2-mercaptoethanol, heated at 100 C for 5 minutes and cooled

Glutamate Glutamate is also a natural amino acid that is produced during the metabolism of L-glutamine and plays an important role as a neurotransmitter in the transmission of sensory perceptions, motor control, as well as learning and memory performance

Fertil Steril 90:16921700 Ronnberg L, Kauppila A, Rajaniemi H (1984) Luteinizing hormone receptor disorder in endometriosis

doi:10.1080/09602011.2021.2011749

Its quinolinium-based structure, synthetic origin and mechanism of action distinguish it from amino acidbased peptides in almost every respect