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To enhance the fluxes to l-cysteine and glycine, we knocked out sdaA to construct the double mutant MG012 by deletion of sdaA in the tnaA mutant MG001

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Establishment of K7M2-Luc orthotopic osteosarcoma mouse model and therapeutic effect evaluation Tumor model establishment To establish orthotopic model, K7M2 cells (3 10 6 cells/well) were dispersed in PBS buffer and implanted into right tibia of BALB/c mice