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It was suggested that insulin from the culture media could be absorbed by and sequestered in these cells [60]

The higher concentrations reduced metabolic activity oxidative phosphorylation, measured using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide, MTT and caused significant leakage of lactate dehydrogenase (LDH) enzymatic activity into the tissue culture medium

For instance, site-specific integration technology is adopted to insert the target gene into a specific site of the bacterial genome, while eliminating potential toxic genes or competing related genes to ensure that the bacteria do not undergo unexpected mutations or gene deletions [204]