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After removing the final wash, the beads were incubated twice at room temperature in 80 l of the digestion buffer (2 M urea, 50 mM Tris-HCl, 1 mM DTT and 0.4 g of trypsin) while shaking at 1,000 rpm

Int J Mol Sci 16(8):1719317230 Panner Selvam MK, Sengupta P, Agarwal A (2020) Sperm DNA Fragmentation and Male Infertility

Pretreatment with JA for alkaline stressed plants mitigated the toxic effects of Na 2 CO 3 and improved the Chl content by 41.26 and 40.11% and carotenoids by 32.43 and 24.00% in 100 mM Na 2 CO 3 + 10 M JA and 150 mM Na 2 CO 3 + 10 M JA treatments, respectively, compared with that of 100 mM and 150 mM Na 2 CO 3 treatments, respectively

declare a filed patent application (WO2019175379 A1 markers of synaptopathy in neurodegenerative disease)