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M.NowickyJ

doi: 10.1016/j.chembiol.2019.01.008 78

Ice crystal formation disrupts the peptides structure, and repeated freeze-thaw cycles have been observed to reduce biological activity by 20-50% (2)

I've also seen many families who have tried leucovorin or paid for it out of pocket, haven't noticed any benefit

However, at therapeutic doses, the net effect of the racemic mix is predominantly that of a cardiac beta 1 stimulant, with little change in peripheral resistance (Westfall et al, 2018)